DNA purification is a crucial step in the sample preparation process. It removes salts and enzymes from the lysed samples, or PCR products, prior to cloning and sequencing. It also removes unwanted PCR-induced artefacts like primer dimers and nucleotides not integrated. DNA purification in molecular biological research is a critical step that requires careful planning to achieve reliable, high-quality results.
There are many different methods to cleaning DNA. The conventional methods for DNA isolation comprise a variety of steps, such as leukocyte separation or red blood cell lysis, to eliminate heme protein inhibitors of the PCR reaction. They also include deproteinization, RNAse treatment, ethanol and isopropanol precipitation, and then finally, DNA elimination. A majority of these procedures require the use of specialized equipment such as an electrophoresis device and a biosafety cabinet due to the hazardous intercalating dyes used in the gel electrophoresis.
Other methods for DNA purification employ spin columns or plates with 96 wells that separate the contaminated particles by adsorbing to the surface. These methods can be quite laborious, especially when working with large amounts of samples or when the columns have to be refilled manually with new reagents.
Dipsticks significantly reduce the number of steps involved in processing samples to only three. They bind nucleic acid using the cellulose-based cellulose wax and release them once water is present. This method is especially beneficial in low-resource http://www.mpsciences.com/2021/04/01/types-of-science-products-available/ settings, like remote field sites as well as teaching labs. Its simplicity and speed (30 s for each sample) makes it ideal for diagnostic molecular applications such as diagnosis as well as genotype screening and heterozygosity testing.